Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, the first of its kind in the State and fourth in the Country, was established by Government of Uttar Pradesh vide U.P. Act No. 27 of 2001 on 25.10.2001 with the College of Veterinary Science & Animal Husbandry, the erstwhile U.P. College of Veterinary Science & Animal Husbandry, Mathura as its main constituent College.

Brij area is known since time immemorial for Lord Krishna and its animal wealth, particularly cows. There are large numbers of Gau-shalas in and around Mathura having hundreds and thousands of cows. The whole of Brij Kshetra is famous for its milk and products (doodh, dahi, makhan, peda etc.). Recognizing the importance of livestock of this area, the erstwhile U.P. College of Veterinary Science and Animal Husbandry was established in Mathura by Government of Uttar Pradesh in 1947.

It was the first Veterinary College in Asia to confer the degree in Veterinary Science. Ever since the establishment of this College, it has contributed significantly not only in terms of number of graduates and post-graduates of high scholastic order, but also through quality research of national and international standards.

This College has a glorious past and the distinction of having its alumni holding high positions in India and abroad as teachers, research workers, policy makers, consultants and administrators.

In the year 1975, this College was made a constituent College of the newly established C.S. Azad University of Agriculture and Technology, Kanpur.

However, keeping in view the requirement of trained and competent manpower in the field of Veterinary Science, Animal Husbandry, Fisheries and other allied disciplines and also to give a fillip to research on different aspects of cattle production, Government of Uttar Pradesh established U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, the fourth Veterinary University in the country.

The Veterinary College, Mathura became the first constituent College of the University. The University is located on the Mathura-Agra Road and is about 5 km from Mathura Junction railway station and 4 km from the new bus stand.

The main campus of the University is spread over a vast land area of 782.32 acres in Mathura Cantt and about 1400 acres at Madhurikund, about 20 km from the main campus.